Plovers abandon successful family to re-nest elsewhere
A successful marriage triggers divorce, at least in plovers. That is because a parent that deserts may achieve higher reproductive success, Naerhulan Halimubieke and colleagues noted.
A bird male and female that successfully raised young had better stick together, you might think, as they have proven to be a good team. And after a disappointing breeding result, it is best for them to split up and find another mate, with which things may go better. Such is the behaviour in most species of birds in which pairs form to breed.
But in plovers, it is the other way round, Naerhulan Halimubieke and colleagues write. A pair of plover parents often will divorce after successfully producing chicks. And after nest failure, male and female stay together to try again. For these birds, this is the best strategy.
The researchers had previously found this pattern – divorce if successful, stick together if failing – in the snowy plover Charadrius nivosus, a ground-nesting bird which lives on sandy beaches. A clutch consists of three eggs in a shallow scrape, which are incubated by both parents. When the chicks hatch, they leave the nest immediately. They find their own food and only need warmth and protection from their parents. A single parent can easily provide this. It is therefore not necessary for both parents to stay with the young until they are completely independent, after about a month.
That is why one of the parents may leave its successful family to find a new mate and initiate another brood. Deserting parents gain time, taking advantage of the breeding season as much as possible; their behaviour results in more offspring on average within a breeding season.
Females desert more often than males, probably because there is a small surplus of adult males, so that on average, females meet a new mate sooner.
A completely different situation arises when a snowy plover brood fails, which in most cases is caused by a predator that detected the nest. The best strategy for the parents in that case is to stay together and start a new nest immediately.
Now, this appears to apply to other species of plovers as well, all of them shorebirds. Halimubieke and colleagues examined eight species. Populations with greater breeding success exhibit a higher rate of divorce within a breeding season than populations with less success, they noted. And within populations, couples with a successful clutch split up more often than couples that see their clutch fail.
Also over years, these birds are not necessarily faithful to their mates. When a new breeding season is coming, they start to nest as soon as possible without worrying too much about mate selection. Having as much offspring as possible – that’s the most important thing.
Willy van Strien
Photo: snowy plover, Charadrius nivosus. Lisa Mcgloin (Wikimedia Commons, Creative Commons CC BY 3.0)
Halimubieke, N., K. Kupán, J.O. Valdebenito, V. Kubelka, M.C. Carmona‑Isunza, D. Burgas, D. Catlin, J.J.H. St Clair, J. Cohen, J. Figuerola, M. Yasué, M. Johnson, M. Mencarelli, M. Cruz‑López, M. Stantial, M.A. Weston, P. Lloyd, P. Que, T. Montalvo, U. Bansal, G.C. McDonald, Y. Liu, A. Kosztolányi & T. Székely, 2020. Successful breeding predicts divorce in plovers. Scientific Reports 10: 15576. Doi: 10.1038/s41598-020-72521-6
Halimubieke, N., J.O. Valdebenito, P. Harding, M. Cruz‐López, M.A. Serrano‐Meneses, R. James, K. Kupán & T. Székely, 2019. Mate fidelity in a polygamous shorebird, the snowy plover (Charadrius nivosus). Ecology and Evolution. 9: 10734-10745. Doi: 10.1002/ece3.5591
Dodder, a plant that parasitizes other plants, flowers almost simultaneously with its host. The parasite takes up the host’s signal that activates flower development, Guojing Shen and colleagues show.
A plant may be covered with a tangle of thin, sticking threads. That is a bad condition for the plant, because those threads are stems of a parasitic plant: dodder (Cuscuta), of which about two hundred species exist worldwide. Most of them thrive on several host plant species. And whether a host flowers sooner or later in the season, dodder joins in and develops its flowers simultaneously. Guojing Shen and colleagues discovered how Australian dodder (Cuscuta australis) manages to synchronize its flowering time with that of its various hosts.
Once young dodder plants get hold of a host plant after germination, their roots disappear, so they cannot take up water and nutrients from the soil anymore. Also, they don’t have green leaves that take carbon dioxide from the air and convert it into carbohydrates with the help of sunlight, like other plants. Everything they need, they extract from the host, round the stems of which they wind extensively.
To exploit its host, the parasite forms numerous haustoria that penetrate into the host’s stems and connect with phloem, the tissue that transports organic compounds, and xylem, the tissue that transports water. The haustoria enable the parasite to extract nutrients and water from its victim.
Annual dodder species, like Cuscuta australis, first grow, then flower and eventually die. The parasite benefits most from its host when it flowers simultaneously. Because if it blooms earlier, it will not reach the size it could have reached by growing longer, and as a consequence it will produce fewer flowers and fewer seeds than it could have produced. But if it postpones flower development for too long, it will be short of nutrients during flowering. Because the host then channels as much nutrients as possible to its own flowers and seeds, leaving less to circulate in phloem and xylem from which dodder taps.
So, dodder has to adjust its flowering time to that of its host.
Dodder is eavesdropping
Most plants regulate their flowering time by tracking changes in day length. When it is about time for flowers to appear, the leaves produce the protein FT (flowering locus T), which moves through the phloem. This protein switches on flower development; it is, in other words, a mobile flowering signal.
Dodder would not benefit from having a flowering signal of its own. As it has to synchronize with its host, it must be flexible. It is therefore not surprising that it does not appear to have functional FT protein. There is a dodder variant of the protein, but it does not activate flowering. How then does the parasite regulate its flowering time?
By eavesdropping on the host’s flowering signal, Shen writes. He investigated flowering in Australian dodder, but the story will apply to other dodder species as well. To European dodder (Cuscuta europaea), for example, which can be found in Western Europe growing on nettle and hops; or to lesser dodder (Cuscuta epithymum), or hellweed, that grows on heather, broom, gorse, thyme and other plants.
It was already known that the parasite not only extracts water and nutrients from the host plant via haustoria, but that also several biologically active substances are exchanged.
Including the FT protein.
As the host starts flower development and the plant produces FT protein, this is transferred to dodder. The researchers show that the host’s protein retains it activity in the parasite, initiating flower development there too.
And so the flowering time of the parasite will coincide nicely with that of its host. Eavesdropping is a perfect method for alignment.
Willy van Strien
Photo: Australian dodder, Cuscuta australis. Harry Rose (Wikimedia Commons, Creative Commons CC BY 2.0)
Shen, G., N. Liu, J. Zhang, Y. Xu, I.T. Baldwin & J. Wu, 2020. Cuscuta australis (dodder) parasite eavesdrops on the host plants’ FT signals to flower. Proceedings of the National Academy of Sciences, online August 31. Doi: 10.1073/pnas.2009445117
Liu, N., G. Shen, Y. Xu, H. Liu, J. Zhang, S. Li, J. Li, C. Zhang, J. Qi, L. Wang & J. Wu, 2020. Extensive inter-plant protein transfer between Cuscuta parasites and their host plants. Molecular Plant 13, 573-585. Doi: 10.1016/j.molp.2019.12.002
During gestation, pot-bellied seahorse males provision the embryos
Seahorses are viviparous, and it is the males that are pregnant. In pot-bellied seahorse, Hippocampus abdominalis, males even provide the embryos with nutrients, Zoe Skalkos and colleagues discovered.
Some fish species are viviparous. In most cases, young fish are born from the mother, but in seahorses the father plays a unique role. He incubates the fertilized eggs in a fleshy, enclosed brood pouch until the offspring can live independently. In daddy’s pouch, the embryos are safe from small predators and pathogens. The pregnant father controls the water quality in the pouch; the highly vascularised pouch skin supplies oxygen and waste products are removed.
When seahorses mate, the female transfers her eggs into her partner’s brood pouch, which he has inflated by filling it with seawater. He fertilizes the eggs immediately and carries them until the young fish can be released. The developing embryos consume the large amount of high protein yolk that the eggs contain.
Pot-bellied seahorse is a large species, up to 35 centimeters long, and exhibits the most complex form of male pregnancy among seahorses. Young embryos are deeply embedded into the pouch’s lining tissue; some are completely covered. The embryos can survive on the amount of food that the yolk contains, according to experiments in which they developed outside a brood pouch. But young fish that are raised in this way exhibit stunted growth and suffer increased mortality. That is why the researchers wondered whether the pregnant father transports nutrients to his hundreds of young via the pouch wall.
To find out, they compared the dry weight of newly fertilized eggs of pot-bellied seahorse with that of newborns, which are released after a gestation period of about 24 days. They also determined the fat content of eggs and newborns. From previous research, they knew that cell constituents that transport fats are produced in large quantities in the brood pouch of males during gestation. Fat is the primary source of energy for the embryos and they need a lot of it.
If the father would not supply nutrients to the embryos, the dry weight of newborn fish would be lower than that of newly fertilized eggs. That is because embryos consume the food supply that the mother provided; they gain weight, but part of the mass is lost by metabolism. The weight loss is estimated to be 30 to 40 percent.
However, as it turned out, newborns have the same dry weight as newly fertilized eggs. Also fat contents were similar. Most likely then, the father provides nutrition to his offspring, especially fats, to replace what is lost.
Pregnant in every sense
Pipefish are closely related to seahorses. Also in pipefish, fathers carry the embryos, although not all pipefish species possess a highly developed, enclosed brood pouch. In some pipefish species, as was known, pregnant males transport a small amount of nutrients to the embryos. Now, this also appears to happen in at least one seahorse species.
These fish dads are going through a pregnancy in every sense. However, compared to that of mammals, their pregnancy is not entirely complete, because the fish mothers still provide most nutrients to the embryos. But it certainly is extraordinary.
Willy van Strien
Photo: Pot-bellied seahorse mating. Elizabeth Haslam (Wikimedia Commons, Creative Commons CC BY 2.0)
The immune system of deep-sea anglerfishes is strongly modified
To the well-known peculiarities of deep-sea anglerfish, Jeremy Swann and colleagues add a new one: some species lack an important part of the immune system. This is associated with a unique parasitic lifestyle of males.
There are strange, very strange and extremely weird animals. We can safely include deep-sea anglerfish in the latter group.
Within the anglerfish or seadevils, they form a separate group of over 160 species, the Ceratioidea, which, as the name indicates, have specialized in living in the utter darkness of the deep sea. Food and partners are extremely scarce down there. Hence, as was known, these fish exhibit some peculiarities. Now, it turns out that they also have a very aberrant immune system, Jeremy Swann and colleagues report.
Angling pole with glowing bulb
Deep sea anglerfish start their lives in a quite normal way, eggs and larvae dwelling in surface waters. But once they developed into young fish, things change. Females grow to a considerable size, males stay tiny.
The bigger a female gets, the more eggs she can produce. And so a young female starts growing. She has to eat much, several prey animals are on her menu. To capture prey, she uses a fishing rod growing from her back; it is a modified anterior dorsal fin. At the end it has a lure: a bulb in which bacteria live that produce light by carrying out a chemical reaction. It is a form of mutualism; the bacteria get a place to live in and food in exchange for light production.
An anglerfish’s light can flash and dance, resembling a moving animal. Living animals discern a tasty snack which they will approach. The anglerfish then ingests a large amount of water, including prey. With some luck, the catch will provide sufficient nutrients to sustain her for quite a while.
The females, plump and with large heads and mouths full of sharp teeth, are not the prettiest of all. They are called seadevils for good reason. They are bad swimmers, drifting around, just waiting for prey to come by.
After completing the larval stage, males’ development takes a completely different direction. Males no longer grow and are unable to eat. Their only goal is to find a female in the empty deep sea. So, they swim constantly. In addition to the light bulb of their angling rod, young females also have two luminous organs on their backs. Maybe the dwarfed males, which have big eyes, are able to detect those organs. If they are lucky, they will meet a partner before they have used up all their reserves.
Upon meeting, he attaches himself to her body with sharp teeth. When, later on, she is ready to release eggs, he is ready to fertilize them. Males and females only become sexually mature when they’ve acquired a partner. Given the scarcity of conspecifics, this makes sense: only after pair formation it is guaranteed that eggs and sperm can come into contact.
In some deep-sea anglerfish, the attachment between female and male is temporary; after a while, he lets go.
But in other species, males attach themselves permanently to a female. These are most bizarre types, because the two partners fuse with each other, enabling the male to survive. Their skin tissues meld, the circulatory systems become connected. He now is a ‘sexual parasite’, little more than a sperm-producing bulge that feeds on nutrients that he derives from her. This sexual parasitism is a unique mode of reproduction, occurring only in deep-sea anglerfish.
It is called parasitism, but it may be considered a form of mutualism as well, as the male delivers sperm in return for nutrients.
The best known species is Ceratias holboelli; it is also the largest one and it has the most extreme sexual dimorphism. A female can grow more than a meter long (including tail), sixty times the size of a free-living male. Physical pair formation is permanent. Once attached to her belly, he grows to a maximum of 20 centimetres. A female carries no more than one parasitic male.
Another species with permanent attachment is Cryptopsaras couesii; in this species, up to eight males can be attached to a single female. A female can be 30 centimetres long, a free-living male only three centimetres.
Ancient immune system
It is remarkable that the female immune system doesn’t attack permanently attached, parasitic males, Swann and colleagues realized. You would expect the immune system to recognize and reject such males, as they are not-own tissue. But that does not happen.
Apparently, the immune system tolerates the very intimate mode of reproduction. To find out how, the biologists examined a number of genes that underpin various parts of immune defence. They investigated four deep-sea anglerfish species with temporarily attached males and six species with permanently attached parasitic males, including Cryptopsaras couesii. They compared these species to a number of anglerfish species outside the deep-sea group, where males don’t attach to females.
Fishes have the same immune system as other vertebrates; the system is 500 million years old. It consists of innate, general immune responses on the one hand and specific immune responses that build up against specific intruders that the system has to deal with on the other hand. The researchers focused on the adaptive, specific immune system.
The results were surprising: deep sea anglerfish species in which males live as parasites on females lack essential immune genes. Their specific immune system is severely blunted.
In two of the species studied, species in which females can have more than one male attached, virtually no specific immune facilities are functional. This is highly remarkable, because such complete lack of specific immune defence is lethal for other animals. The first infection would kill them. Microbial pathogens occur in the deep sea too, so deep-sea anglerfish must be able to defend themselves. Most likely, they reorganized their innate immune defence, the researchers assume.
From their own and other research, they conclude that the common ancestor of deep-sea anglerfish had tiny, non-parasitic males that temporarily attached themselves to females. On a few occasions, species descending from that ancestor made the switch to permanent attachment and their specific immune defence has been largely dismantled.
It is unclear yet what happened first. Did males become parasitic, making it necessary to turn off the specific immune system? Or did the specific immune system lose important parts, making permanent attachment of males possible?
The deep sea anglerfish remain really puzzling creatures.
Willy van Strien
Drawing: northern giant seadevil, Cryptopsaras couesii (not included in this research); female with male attached. Tony Ayling (Wikimedia Commons, Creative Commons CC BY-SA 1.0)
Swann, J.B., S.J. Holland, M. Petersen, T.W. Pietsch, T. Boehm, 2020. The immunogenetics of sexual parasitism. Science, online July 30. Doi: 10.1126/science.aaz9445
Fairbairn, D.J., 2013. Odd couples. Extraordinary differences between the sexes in the animal kingdom. Princeton University Press, Princeton and Oxford, VS. ISBN 978-0-691-14196-1
Parasitic Vidua nestlings trick host parents with near-perfect mimicry
In order not to stand out in the nest in which they grow up clandestinely, Vidua nestlings mimic the young of the host parents. They perform very well, Gabriel Jamie and colleagues report. But some slight discrepancies exist.
African whydahs and indigobirds, Vidua species, are brood parasites like the cuckoo. They lay their eggs in the nest of other bird species, in this case grassfinches, and have the host parents raise their young. Vidua finches are unable to provide parental care. But these brood parasites do much less harm to the host families than a cuckoo, because young Viduas don’t eject other nestlings from the nest. The host parents take care for their own offspring, but have some extra, foreign young.
The foreign nestlings should not stand out, otherwise the tricked parents will notice the deception. It was already known that Vidua young resemble their host parents’ young. With special computer software, Gabriel Jamie and colleagues now show how successful the mimicry is.
The Vidua genus contains nineteen species. In the breeding period, the males are real beauties, while the females are inconspicuous and difficult to recognize. Jamie took a closer look at three species: pin-tailed whydah (Vidua macroura), broad-tailed paradise whydah (Vidua obtusa) and purple indigobird (Vidua purpurascens). They are host-specific, each Vidua species has a single host species. Jamie compared the Vidua nestlings to that of their respective host parents and of a number of other grassfinch species.
Young grassfinches (Estrildidae) have ornamental mouth markings that become fully visible when they open their beaks; this ornamentation in unusual among birds. Each grassfinch species has its characteristic pattern, colour and structure.
Nestlings of the breeding parasites accurately mimic those characteristic markings, is the conclusion of the research. An analysis with pattern recognition software shows that the pattern is similar to that of their host parent species. The colours match well too. Vidua nestlings also cleverly imitate the begging calls and postures of their foster siblings.
Previous research, by Michael Sorenson, had shown that the nineteen species of whydahs and indigobirds are much younger than their hosts in an evolutionary sense. The idea is that their common ancestor switched to a brood parasitic lifestyle with a grassfinch as host parent.
Speciation could then occur quickly. Whenever a Vidua female happens to lay eggs in the nest of another host, a separate group associated to that new host arises, because Vidua nestlings imprint on the song of their host father. Each grassfinch species has its own characteristic song. When grown up, Vidua males will mimic the song of their host, and females are attracted to this song. Also, females select a nest of the host species they were raised by to lay their eggs in. The group turns into a new species.
The nestlings then become more and more similar to the nestlings of the new host through an evolutionary adaptation process. Because the more a Vidua nestling resembles the young of its host parents, the more likely they are to accept it and care for it, increasing its survival chance.
And as a matter of fact, the resemblance between foreign and own young in a parasitized grassfinch’s nest turned out to be striking. But it is not entirely perfect. Small but consistent differences exist. Perhaps the foreign nestlings are (yet) unable to fully mimic their nest mates. And apparently, they are doing well enough: the host parents accept them.
But there may be another explanation for the discrepancies, the researchers write. Nestlings of pin-tailed whydah, for example, have spots in the beak that are slightly larger than those of their foster parents’ young, common waxbill (Estrilda astrild), and their begging calls are slightly extended. Unlike a waxbill nestling, they wave a wing under their open mouth while begging.
So, these Vidua nestlings are slightly exaggerating their host’s begging signals. And perhaps the host parents favour them as a consequence. An intriguing thought.
Courting blue-capped cordon-bleu stamps its feet while bobbing
In blue-capped cordon-bleu, male and female show their commitment with song and movement. With a high-speed camera, Nao Ota revealed the tap dance that is hidden within.
The complex courtship displays of the blue-capped cordon-bleu, an estrildid finch species from East Africa, are nice to observe. Holding a piece of nesting material in the beak, the bird is singing and bobbing up and down. What we don’t see is that it rapidly stamps its feet several times during bobbing. Nao Ota made that ‘tap dance’ visible by recording the courtship with a high-speed camera. Earlier, she had filmed birds in the lab, now she also has footage from the field.
The birds live in monogamous pairs. Male and female look similar, but the male has more blue plumage than the female. Both sexes give song and dance performances.
Blue-capped cordon-bleus perform most intensively when their mate is present on the same perch, but they don’t perform a duet. In contrast to humans, they may be able to see the fast tapping. Feet stamping produces sound, as Ota had already shown, and the birds probably feel the vibrations it causes in the perch on which they are sitting.
In presence of a conspecific bird besides the couple, they sing and dance more frequently. The performing bird then points its tail towards its mate. This seems to mean that the display is directed to the partner and to express commitment.
Willy van Strien
Photo: Blue-capped cordon-bleu, Uraeginthus cyanocephalus, male. Peter Steward (via Flickr. Creative Commons CC BY-NC 2.0)
Ota, N., 2020. Tap dancers in the wild: field observations of multimodal courtship displays in socially monogamous songbirds. The Science of Nature 107: 30. Doi: 10.1007/s00114-020-01686-x
Ota. N., M. Gahr & M. Soma, 2018. Couples showing off: Audience promotes both male and female multimodal courtship display in a songbird. Science Advances 4: eaat4779. Doi: 10.1126/sciadv.aat4779
Ota. N., M. Gahr & M. Soma, 2017. Songbird tap dancing produces non-vocal sounds. Bioacoustics 26: 161-168. Doi: 10.1080/09524622.2016.1231080
Ota. N., M. Gahr & M. Soma, 2015. Tap dancing birds: the multimodal mutual courtship display of males and females in a socially monogamous songbird. Scientific Reports 5: 16614. Doi: 10.1038/srep16614
Bird doesn’t attack the spider, but its web decoration instead
Thanks to a striking web decoration, the spider Cyclosa monticola escapes from hungry birds, as Nina Ma and colleagues show. The birds’ attacks fail.
The spider Cyclosa monticola, which is common in East Asia, constructs an elaborate web. Not only does it consist of sticky threads, but it also carries a striking, linear band of trash, containing moults, prey remains, and pieces of leaves and stems. According to Nina Ma and colleagues, this detritus decoration diverts attacks of predators, especially birds, away from the spider.
The spider is in the centre of its web, hardly visible in the decoration band, which extends in both directions. The animal’s colour is similar to the colour of the decoration. Birds cannot distinguish the colours of the spider from that of decorating trash.
The researchers wondered whether Cyclosa monticola, a tasty snack for many birds, is safer as a result. In order to find out, they exposed spider webs to domestic chickens chicks, one web per chick. Some chicks were given a web with the resident spider present in it; from half of these webs, the researchers had removed the decoration with fine scissors without damaging the web. For comparison, other chicks were given either an empty web or a web with decoration only. Most chicks quickly pecked at whatever (spider and/or decoration) they saw. The researchers were interested in their first target.
Spiders that had been robbed of their web decoration were grabbed by the chick in almost all cases. Spiders that had kept their decoration survived much more frequently. In this situation, the chick usually did not peck at the spider, but at the trash, and the spider dropped down quickly to escape. Obviously, the decoration did confer safety.
Although the spider is hardly detectable among the decoration trash, the protection was not only due to camouflage, as the researchers argue. Because in that case, attacks would be random and the risk for the spider to get captured would be equal to its size relative to the size of the decoration. However, the chance of being captured was lower and independent of the size of the decoration band. Apparently, the detritus decoration is more attractive to birds to peck at and diverts their attention away from the spider.
So, web decoration is an effective defence strategy.
The question now is whether insect prey will not avoid the structure. After all, the trick of a spider web is that insects don’t discern the threads and get caught in the web. But research on another spider species that adorns its web shows that insects are attracted to the decoration – and get stuck in the web. The web decoration of Cyclosa monticola may also have this effect; if so, it would have a double function.
Ma, N., L. Yu, D. Gong, Z. Hua, H. Zeng, L. Chen, A. Mao, Z. Chen, R. Cai, Y. Ma, Z. Zhang, D. Li, J. Luo & S. Zhang, 2020. Detritus decorations as the extended phenotype deflect avian predator attack increasing fitness in an orb‐web spider. Functional Ecology, online July 16. Doi: 10.1111/1365-2435.13636
Tan. E.J., S.W.H. Seah, L-M.Y.L. Yap, P.M. Goh, W. Gan, F. Liu & D. Li, 2010. Why do orb-weaving spiders (Cyclosa ginnaga) decorate their webs with silk spirals and plant detritus? Animal Behaviour 79: 179-186. Doi: 10.1016/j.anbehav.2009.10.025
When the eggs are brown-coloured, hoopoe male is more helpful
A brooding hoopoe female repeatedly smears a dark substance from her preen gland onto the eggs. The browner the eggs, the more helpful her partner will be. That is because the colour has meaning to him, Silvia Díaz Lora and colleagues think.
During incubation, a dark-coloured, foul-smelling substance is produced in the preen gland (uropygial gland) of hoopoe females, which is greatly enlarged during this period. It was already known that a female smears the material onto the eggs with her beak. The colour that the eggshells get as a result determines how frequently the male will feed her, Silvia Díaz Lora and colleagues now report.
This has to do with the hatching success of the clutch that is to be expected. If prospects are good, he will invest a lot. If not, he will save energy for the next breeding event. The egg colour is an indication of the expected success.
In the breeding season, hoopoes establish a territory, form pairs and breed in tree cavities. Both parents take care of the nestlings until they fledge. But before nestlings appear, the clutch has to be incubated, which is the females’ task. They don’t leave the nest during this period, and they also stay with the young during the first week. Their partners provide them with food.
The preen gland secretion that the females spread onto their eggs protects the embryos, thanks to bacteria that live in the gland during the breeding period and produce compounds that inhibit pathogenic bacteria. When an egg is covered with gland secretion, pathogens are prevented from penetrating the egg shell. This increases the chance the eggs will hatch, and the higher the density of beneficial bacteria, the better the result.
The mother smears the antimicrobial substance onto each egg soon after laying and repeats the procedure until the young hatch. Microscopic cavities on the eggshell enhance adhesion of the substance. Newly laid eggs are light bluish-gray, but they turn dark and greenish-brown by the treatment.
Embryos of other bird species are protected by an extra layer in the egg. But the hoopoe has its own, unique way.
Later on, also the nestlings’ preen gland will produce the brown secretion. The substance has another function: the stench deters predatory enemies. Outside the breeding season, the preen gland of females and fledged young, like that of males, is small. The bacteria are disappeared and the gland produces a white, colourless fat, which the birds use to preen their plumage.
Among hoopoe females, there is a difference in the colour of the substance they paint the eggs with. And what is important for this story: that difference is related to the amount of bacteria that are present in the preen gland. Without bacteria, the substance is red, with bacteria it is brown. The browner the colour, the higher the bacterial density – and the stronger the antimicrobial activity.
That means: the browner the eggs, the better the embryos are protected against infections.
When bringing food to their mate, males see the colour of the eggs. The researchers wanted to know whether they adjust their feeding effort to this colour. On the basis of video recordings at a number of nests, they investigated at what frequency the male came to the nest and what prey he carried. With a spectrometer, they measured the colour of the clutch and they sampled the female’s preen gland contents to assess the density of bacteria.
Results are appealing. If the eggs were brown-coloured, the male frequently fed his partner while she was incubating. If the eggs were more reddish, he only worked this hard if her body condition was good. Apparently, hoopoe males are willing to invest a lot in a clutch if it is promising because the mother colours the eggs with a potent antibacterial material or because she is healthy.
Once the eggs had hatched, father’s behaviour no longer depended on the egg colour. There are probably other factors that determine his diligence at that stage, such as the begging behaviour of the young.
But there is no proof yet that this story – if eggs are brown, a hoopoe male will work harder – is really true. An alternative possibility is that a father who brings in more food for its partner simply has a richer territory than a father who brings less. Thanks to lots of available food, the mother is in better condition and able to maintain a larger population of bacteria in her preen gland, which colours the secretion brown and gives the eggs good protection. So: the quality of the territory may determine both how much food the male brings in and the colour of the eggs.
To find out what is true, the researchers would have to do experiments in which they paint hoopoe eggs darker and see whether males respond by working harder. For now, it looks like they adjust their effort to the chance that the eggs will hatch successfully. But only if such experiments corroborate this, we can be sure.
Willy van Strien
Photo: Eurasian hoopoe, Upupa epops. Imran Shah (Wikimedia Commons, Creative Commons CC BY-SA 2.0)
Díaz Lora, S., T. Pérez-Contreras, M. Azcárate-García, M. Martínez-Bueno, J.J. Soler & M. Martín-Vivaldi, 2020. Hoopoe Upupa epops male feeding effort is related to female cosmetic egg colouration. Journal of Avian Biology, online June 20. Doi: 10.1111/jav.02433
Martín-Vivaldi, M., J.J. Soler, J.M. Peralta-Sánchez, L. Arco, A.M. Martín-Platero, M. Marínez-Bueno, M. Ruiz-Rodríguez & E. Valdivia, 2014. Special structures of hoopoe eggshells enhance the adhesion of symbiont-carrying uropygial secretion that increase hatching success. Journal of Animal Ecology 83: 1289-130. Doi: 10.1111/1365-2656.12243
Soler, J.J., M. Martín-Vivaldi, J. M. Peralta-Sánchez, L. Arco & N. Juárez-García-Pelayo, 2014. Hoopoes color their eggs with antimicrobial uropygial secretions. Naturwissenschaften 101: 697-705. Doi: 10.1007/s00114-014-1201-3
Thanks to black irises, female guppy escapes from predator
By drawing the attention of a predatory fish to the eyes and turning the head away as soon as it strikes, a guppy female manages to evade. Robert Heathcote and colleagues report this hitherto unknown escape strategy.
When Trinidadian guppies detect a predatory fish, they will approach and inspect it to find out if it is hungry and dangerous. The colour of their irises may change when they do; normally the irises are silver, but then they often turn black, making the eyes more salient. It doesn’t seem profitable to draw an enemies attention to the head, so Robert Heathcote and colleagues wondered why guppies blacken their eyes. Is it to deter the enemy? Or is it to divert its attack? But then, how does it work?
Wild Trinidadian guppies, Poecilia reticulata, live in northeastern South America. One of their enemies is the cichlid Crenicichla alta, a predatory fish that ambushes its victims.
First, the researchers exposed wild guppies to a visually-realistic model of this predatory fish in a tank, and observed whether they blackened their eyes. As it turned out, large individuals do. These are mostly females, which are larger than males on average.
The predatory fish is not deterred by those dark eyes, as became evident from the next series of experiments, this time with live predatory fish and models of guppies with either black or silver irises. The cichlid attacks guppies with black eyes as often as those with silver-coloured irises. So, the first possible explanation fails.
The researchers also investigated at what target the predator lunges when attacking its prey. When irises are silver-coloured, the predator aims at the broadest part of the body, they discovered. In dark-eyed fish, the attack is diverted to the front. So, colour change of the irises appears to be a diversion strategy. But the predatory fish grasps both types of models, with black and silver irises, just as easily, so a guppy doesn’t benefit from a dark eye colour in itself.
However, colour change does help when combined with a critically timed evasive manoeuvre, as the last tests showed. In these tests, living guppies were placed in a tank with a living cichlid, but were separated from it by a transparent acetate screen, keeping them from danger. From the movements of the fish, filmed with a high-speed camera, the researchers were able to calculate, for each attack, the probability that the predator would have caught the victim in real conditions, without screen.
The moment the predatory fish strikes, a guppy quickly pivots around an imaginary vertical axis, accelerates and swims away. The imaginary axis runs through the broadest part of the body (more precisely, through the centre of mass), roughly the point that the cichlid aims for in a victim with silvery, less conspicuous irises. This part of the body hardly moves during the rotation. If the predatory fish directs its attack at that point, its chances to succeed are high, as the analysis showed.
The head, on the other hand, immediately leaves its position during the rotational movement. If the predatory fish charges at that part of the victim – like it does in a prey with black irises – it usually misses out.
By blackening its eyes, a guppy thus increases its chance to escape with a quick manoeuvre. The researchers compare this escape strategy – drawing the enemy’s attention to a particular point and then moving it quickly away – with the behaviour of a bullfighter, the matador with his red cape. Such escape strategy was previously unknown in animals.
For the strategy to be successful, the distance between the eye and the broadest part of the body must be sufficiently large. Males are too small. Males also have a striking eye-sized black spot on their body, which makes it more difficult to draw the predator’s attention to the head. So it makes no sense for males to blacken their irises in presence of a predatory fish, just increasing the detection risk. Accordingly, they don’t.
But females can trick their enemies by making their eyes stand out. The predator aims for her attractive eyes. And they’re gone.
Willy van Strien
Photo: Guppy, Poecilia reticulata, female with silver iris. H. Krisp (Wikimedia Commons, Creative Commons, CC BY 3.0)
Heathcote, R.J.P., J. Troscianko, S.K. Darden, L.C. Naisbett-Jones, P.R. Laker, A.M. Brown, I.W. Ramnarine, J. Walker & D.P., 2020. A matador-like predator diversion strategy driven by conspicuous coloration in guppies. Current Biology, online June 11. Doi: 10.1016/j.cub.2020.05.017
When a ladybird has to defer predators regularly, it is less able to resist pathogens and parasites, Michal Knapp and colleagues write.
When threatened, ladybird beetles try to avoid being eaten by excreting a yellow, smelly and bitter-tasting liquid from their legs. This reduces the appetite of hungry insects, lizards, birds or small mammals. The liquid is haemolymph, the insect variant of blood. You can see the phenomenon by provoking a ladybird.
They conducted experiments with the harlequin ladybird, Harmonia axyridis. The species originally lived in East Asia, was introduced in Europe and North America and nowadays also occurs in South America and Africa.
Haemolymph is an expensive means to scare away enemies. It contains nutrients, as well as blood cells, proteins and other compounds that ladybirds need to eliminate pathogens and parasites. The harlequin ladybird uses, among other compounds, the substance harmonine, which has a strong antimicrobial effect. Each bleeding causes a loss of these valuable components.
To measure the effect of this loss, Knapp triggered reflex bleeding in ladybirds twice a week, during three weeks. Contrary to his expectations, the treatment did not affect the survival of the beetles, and they did not lose weight.
He also, during a month, triggered newly hatched females daily to bleed, and found that their reproductive capacity was unaffected. In their first month of life, they produced as many eggs as females that were untreated. They started laying eggs a few days later, though, especially after losing a high volume of haemolymph. That may be of little importance, however, as the beetles live for months.
But bleeding, the defence mechanism against predators, comes at the expense of the resistance to other enemies, as it turned out. The concentration of blood cells and proteins in haemolymph had decreased. The concentration of harmonine and similar compounds has not been measured, but other research indicates that it also will have decreased.
Indeed, haemolymph of ladybirds that bled was found to inhibit bacteria less strongly. Probably, these ladybirds are less resistant to parasites as well, as blood cells take part in defence, but this has not been investigated.
Ladybirds successfully deploy constituents of haemolymph against all types of enemies – but they cannot fight them all at once at full power. If they have to deal with hungry predators frequently, their resistance to pathogens and parasites is reduced.
Knapp. M., M. Řeřicha & D. Židlická, 2020. Physiological costs of chemical defence: repeated reflex bleeding weakens the immune system and postpones reproduction in a ladybird beetle. Scientific Reports 10: 9266. Doi: 10.1038/s41598-020-66157-9